By Donald Emmeluth
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Extra resources for Botulism (Deadly Diseases and Epidemics) 2nd Edition
After a wonderful night’s sleep, Jim woke feeling refreshed but still quite full from the night before. He decided to take a jog and get some exercise. Later that afternoon, Jim decided to take a ride around the Savannah area and told his grandmother he would get something to eat in town. When Jim returned to his grandmother’s house later that evening, he noticed that her car was gone. It seems that his grandmother had taken Mrs. O’Riley to the local hospital. The answering machine contained a message from his grandmother with directions to the hospital.
Several new surface layers develop outside the core (cell) wall, including the cortex and spore coat. The cytoplasm is dehydrated and contains only the cell genome and a few ribosomes and enzymes. The endospore is cryptobiotic (exhibits no signs of life) and is remarkably resistant to environmental stresses such as heat (boiling), acid, irradiation, chemicals, and disinfectants. Some endospores have remained dormant for 25 million years preserved in amber, only to be shaken back into life when extricated and introduced into a favorable environment.
In June 2009, the unthinkable occurred and Nestles’ chocolate chip cookie dough was found to contain E. coli O157:H7, causing the hospitalization of at least 25 people, seven of those suffering with a severe type of kidney failure. Transmission of Botulism Finding the potential source of the contamination has become an exercise in molecular biology dependent upon high-quality extraction of the DNA, RNA, and proteins of the suspected pathogens. Unfortunately, some of the current extraction methods such as heat, electrical charge, and chemical separation can alter or destroy the very molecules that are being sought.
Botulism (Deadly Diseases and Epidemics) 2nd Edition by Donald Emmeluth