By Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)
Confocal microscopy with laser scanning expertise yields in-vivo photographs of ocular and ocular adnexal surfaces which are so significant that they rival histology by way of quality.This distinctive atlas and textbook demonstrates common in-vivo anatomy of the cornea, limbus and conjunctiva, quantifies a number of mobile buildings utilizing cell-density calculations and establishes correlations among novel optical sections of varied illnesses of the ocular floor and scientific findings. additionally, it helps the translation of novel high-magnification optical sections by way of evaluating corneal and conjunctival imprint cytology with in-vivo pictures and describes early inflammatory adjustments in corneal grafts, in addition to corneal conjunctivalisation in limbal stem mobile deficiency, corneal dystrophies or infections, flap interface and margin features after laser in-situ keratomileusis (LASIK). furthermore, it instructs the reader approximately diagnostic and healing follow-up suggestions and gives a quick advent to purposes in different fields equivalent to dentistry and ear, nostril and throat surgery.
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Extra info for Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology
Ex vivo conditions enable the process of image acquisition to be optimized and standardized, thus yielding optimal resolution and contrast. The information obtained in this way may be helpful when similar structures have to be identified and interpreted under in vivo conditions. The following examples illustrating blood constituents, pathogenic microorganisms, and ocular tissue structures demonstrate the resolution of the RCM under optimized experimental conditions. 1 Blood Components Fig. 1 Ex vivo: Blood, concentrated; rouleaux phenomenon 26 Chapter 4 Ex Vivo Applications a b c d Fig.
B Impression cytology from the same pa- b to conjunctival cells. 2 Epithelium a Fig. 31 Corneal epitheliopathy. a Slit-lamp photograph of a patient with severe ocular rosacea and conjunctival cell migration within the corneal epithelium. b Confocal in vivo microscopy image from the b same patient. Conjunctival cells (hyperreflective cells) migrating into an abnormal corneal epithelium (hyporeflective cells) a b Fig. 32 Corneal conjunctivalization in a 55-yearold woman as a complication of severe ocular rosacea.
1 Noncontact microscopy. Scanning laser reflex (arrow) on the cornea (argon laser/Heidelberg Retina Angiograph Classic) Fig. 2 Normal tear film 32 Chapter 5 Confocal Laser Scanning In Vivo Microscopy Replacing the contact system in the confocal laser scanning microscope with a dry objective lens, combined with an 80–90% absorption gray filter to decrease laser intensity (Fig. 1), enables the fine structure of the tear film to be imaged (Fig. 2). The rapid imaging sequence in the device also permits dynamic processes to be recorded [51, 77, 88].
Atlas of Confocal Laser Scanning In-vivo Microscopy in Ophthalmology: Principles and Applications in Diagnostic and Therapeutic Ophthalmology by Prof.Dr. med. Rudolf F. Guthoff, Christophe Baudouin MD, Phd, Prof.Dr. rer. nat. Joachim Stave (auth.)